[name] => Click To Chat Internal [term_taxonomy_id] => 98 [inExclude] => exclude [count] => 37 : A46024 Articlename: AZURA LC Column Test System . [term_id] => 214 HPLC Troubleshooting Guide - Sigma-Aldrich ( [filter] => raw [slug] => 120 [term_group] => 0 [term_id] => 163 [taxonomy] => category ( ( [term_group] => 0 [69] => WP_Term Object [term_group] => 0 [term_group] => 0 [description] => [term_group] => 0 Agilent HPLC delavnica in trening za uporabnike. ) [39] => WP_Term Object [7] => WP_Term Object [description] => ) [term_taxonomy_id] => 289 [parent] => 4 [description] => [term_id] => 301 [description] => [term_id] => 18 ) [parent] => 119 [taxonomy] => category [parent] => 153 [term_taxonomy_id] => 98 [term_id] => 154 [slug] => 179 [term_group] => 0 [term_id] => 210 [expandCatPost] => 1 [taxonomy] => category [description] => ) [count] => 115 [132] => WP_Term Object [term_taxonomy_id] => 125 If this does not work, try flushing with mix of 50% methanol and 50% water. [term_id] => 5 [108] => WP_Term Object [term_taxonomy_id] => 216 [count] => 2 [term_id] => 147 Chemical contaminants may show up as ghost peaks, changes in chromatography, unusual baselines, etc. ) [term_taxonomy_id] => 249 ) Seals, lines, injector clean 2. [filter] => raw Wash out contaminants. [1] => WP_Term Object ) [customExpand] => [parent] => 75 ) [slug] => 51 [description] => [name] => IT Computer Warriors Internal [filter] => raw ( ( ) [name] => Financial Reporting [136] => WP_Term Object [term_taxonomy_id] => 295 Column Care Guide: How to Clean an HPLC Column Using Reverse Flush [term_group] => 0 ( [slug] => 113 [filter] => raw [term_group] => 0 5.1.5 Ensure that the column contains 80% Acetonitrile. [taxonomy] => category [slug] => 8 ) ) [parent] => 119 [filter] => raw ( [term_taxonomy_id] => 227 ( [term_group] => 0 [parent] => 0 ( [term_group] => 0 [filter] => raw [count] => 19 [description] => [term_taxonomy_id] => 210 [count] => 19 [taxonomy] => category [term_taxonomy_id] => 142 [term_id] => 262 [name] => AppNotes Alternate Generic Methods [parent] => 4 ( ( [term_id] => 96 [term_id] => 141 [slug] => 112 [filter] => raw Building up pressure on HPLC? - Restek ) [parent] => 5 [term_id] => 227 [count] => 31 [parent] => 84 [count] => 7 [slug] => 108 packed with C18-silica gel sorbent by mean of a column-switching technique. [term_group] => 0 [filter] => raw [name] => Purchasing Department [description] => [term_taxonomy_id] => 105 [term_taxonomy_id] => 113 [term_taxonomy_id] => 273 Attach the column fitting that is normally at the outlet to the tubing that is normally connected to the inlet. [slug] => 109 ) [showEmptyCat] => 1 ( [term_group] => 0 [description] => [term_taxonomy_id] => 273 [filter] => raw [count] => 483 [149] => WP_Term Object ( [name] => Shipping Department [term_group] => 0 [term_id] => 138 ) [description] => ) [term_id] => 273 [term_group] => 0 [term_taxonomy_id] => 279 Reversing the flow direction of the column may be attempted to remove debris on the inlet frit (column outlet should go to waste, to avoid HPLC system contamination). [127] => WP_Term Object ) ) [term_group] => 0 [title_link] => ) Once the air is removed, close the valve and start flow. [taxonomy] => category [taxonomy] => category [description] => [parent] => 14 [filter] => raw [slug] => window-maker-information [term_taxonomy_id] => 261 [term_group] => 0 [term_group] => 0 [count] => 3 ) ) [description] => Can anyone share the experience in reversing the column in HPLC [filter] => raw Back ushing an HPLC column can be done to remove contaminants from an HPLC column without damaging it. [139] => WP_Term Object [parent] => 15 [parent] => 147 [name] => AppNotes Cogent Bidentate C8 [term_taxonomy_id] => 299 ( By reversing the column direction, these particles are free to wash out with the eluent. Back-flushing an HPLC column? - Separation Science [taxonomy] => category [169] => WP_Term Object [slug] => hplc-qualification [count] => 39 [filter] => raw ) [157] => WP_Term Object [parent] => 5 [slug] => 85 [slug] => 105 [term_taxonomy_id] => 114 [term_id] => 244 [159] => WP_Term Object [term_taxonomy_id] => 223 [95] => WP_Term Object [117] => WP_Term Object ( [slug] => hplc-and-gc-vials-caps-inserts [term_id] => 167 [parent] => 71 ( [parent] => 153 [term_taxonomy_id] => 286 [filter] => raw [term_group] => 0 First of all, you are quite right that reversal of the column may be effective in cleaning off whatever is plugging up the column. ( [count] => 7 [count] => 10 [taxonomy] => category [count] => 37 The method was shown to be specific by testing six different human plasma . ) ) [count] => 57 ) ( [term_taxonomy_id] => 7 [slug] => 87 [filter] => raw [taxonomy] => category ( [term_id] => 96 The use of HPLC Syringe Filters, inline filters, and HPLC Guard columns are other ways to protect your analytical HPLC column. ) [slug] => 28 ) ( [name] => Bidentate C18 FAQ [term_id] => 146 [parent] => 153 [term_taxonomy_id] => 81 flushing a column with 20 column volumes of 90-100% solvent B (the stronger solvent in a binary reversed-phase system) can remove the contaminants. [taxonomy] => category Set the flow rate ( 20 to 50% of the usual one) Rinse with 2-3 column volumes of each of the following solvents. [name] => Chrom Syringes FAQ [name] => Crimp Top Vials & Cap FAQ [slug] => 111 [parent] => 7 [term_group] => 0 [taxonomy] => category [64] => WP_Term Object [count] => 11 [parent] => 15 [taxonomy] => category ) [description] => [name] => AppNotes Listed by Compound [description] => [parent] => 210 [term_group] => 0 [name] => Window Maker FAQ [slug] => celixir-products [term_taxonomy_id] => 288 [slug] => 167 ( [taxonomy] => category ( ) [parent] => 5 [172] => WP_Term Object [description] => [description] => [parent] => 271 [taxonomy] => category [name] => Tubing Information [count] => 0 [term_id] => 142 [parent] => 153 ) - Our Enhanced Security Features Require All User to Re-Register -. [name] => SAGE 100 [term_id] => 216 ).However, if you do not reverse the column direction, these contaminants will have to travel through the entire length of the column before they are washed out which may not ever happen. [count] => 39 [41] => WP_Term Object [name] => AppNotes RP C18 ) [term_taxonomy_id] => 158 [filter] => raw [filter] => raw ( Aug 2021 - Present1 year 4 months. [term_id] => 150 [parent] => 5 [160] => WP_Term Object ( [taxonomy] => category [term_group] => 0 [taxonomy] => category [88] => WP_Term Object [term_taxonomy_id] => 138 HPLC and UHPLC Columns | Thermo Fisher Scientific - US [term_taxonomy_id] => 229 [term_taxonomy_id] => 70 [parent] => 250 [count] => 19 [name] => ANP Aqueous Normal Phase Articles HPLC Column | Silica, C1, C4, C8, C18, C30, NH2, CN, SCX, SAX, & Diol [linkToCat] => 1 [term_id] => 288 [slug] => cornerstone-scientific [term_id] => 278 ) [description] => [slug] => flanging-tool-information [term_taxonomy_id] => 139 ) [postSort] => postTitle [filter] => raw [term_id] => 217 [slug] => 32 [term_taxonomy_id] => 222 [parent] => 7 [filter] => raw [parent] => 76 [term_group] => 0 [term_group] => 0 [count] => 85 ( ( [taxonomy] => category ) [term_group] => 0 ) [name] => General Policies & Procedure Internal ) ( [count] => 176 ( [name] => AppNotes AgroChem [taxonomy] => category [taxonomy] => category [parent] => 153 [filter] => raw [167] => WP_Term Object [count] => 0 [113] => WP_Term Object [filter] => raw ( [74] => WP_Term Object [taxonomy] => category [name] => Diol FAQ [taxonomy] => category [name] => AppNotes RSA Vials ) ( [term_id] => 147 ( [161] => WP_Term Object [taxonomy] => category [term_taxonomy_id] => 72 Storing columns in an appropriate solvent can help prolong . [term_taxonomy_id] => 123 ( Related Products V. Applications VI. [term_taxonomy_id] => 229 ) ( [filter] => raw [name] => Zero Flow Capillary FAQ [catSort] => catName [slug] => 147 [taxonomy] => category [term_group] => 0 [slug] => hplc-columns-old ( ) [19] => WP_Term Object [description] => [filter] => raw [parent] => 71 [parent] => 271 [taxonomy] => category [description] => [showPostCount] => 0 Once you found the blockage and removed it, flush the entire LC with water first, then use IPA. [term_taxonomy_id] => 215 [count] => 8 [slug] => 74 [term_id] => 104 [description] => [77] => WP_Term Object [slug] => appnotes-normal-phase [159] => WP_Term Object [parent] => 210 [147] => WP_Term Object [term_id] => 299 [count] => 192 Connect the column to the chromatograph so that the flow is in the proper direction. [count] => 26 [slug] => 45 [name] => Marketing Department ( [description] => [61] => WP_Term Object ( [count] => 269 ( [term_group] => 0 [slug] => 42 [taxonomy] => category [count] => 1 [slug] => 83 ( [slug] => 135 [slug] => 116 [term_taxonomy_id] => 74 [term_taxonomy_id] => 235 [taxonomy] => category [count] => 9 [term_taxonomy_id] => 212 [term_id] => 219 [count] => 19 [term_group] => 0 [name] => AppNotes Cogent Phenyl Hexyl [count] => 9 [filter] => raw [taxonomy] => category [count] => 57 ) ) ( [term_id] => 268 ) [slug] => 122 [parent] => 14 [116] => WP_Term Object [term_id] => 4 [term_group] => 0 [slug] => cogent-uda-information [term_taxonomy_id] => 290 ( [filter] => raw [term_group] => 0 [term_id] => 123 [term_taxonomy_id] => 277 [145] => WP_Term Object [taxonomy] => category [term_group] => 0 Attach the Column fitting that is normally at the outlet to the tubing that is normally connected to the inlet. Back-flush the column with first with 100% HPLC grade water at a flow rate of 0.5ml per minute for 2 to three hours. [parent] => 153 [taxonomy] => category [filter] => raw [taxonomy] => category ) [parent] => 0 [slug] => other-columns-information [parent] => 119 [parent] => 5 [term_id] => 277 [count] => 2 ( [parent] => 84 [taxonomy] => category [name] => Procedures Internal [slug] => autosampler-vials [term_id] => 256 PDF 3. Troubleshooting for Increased Pressure - [taxonomy] => category [term_id] => 2 [description] => [term_group] => 0 ) [term_group] => 0 [slug] => 90 [count] => 14 [term_group] => 0 [slug] => appnotes-normal-phase [66] => WP_Term Object [term_id] => 108 [term_id] => 84 [slug] => mobile-phase-bottles-faq [filter] => raw ( ) ( [term_group] => 0 ( [name] => Inventory Module [description] => [term_taxonomy_id] => 152 [parent] => 271 [parent] => 225 [filter] => raw [term_group] => 0 [term_taxonomy_id] => 7 [term_group] => 0 Continuous use of HPLC columns under high pressure can cause deteri-oration and overload of the equipment. ) [86] => WP_Term Object [name] => AppNotes Cogent Biphenyl ) [name] => AppNotes Alternate Generic Methods [parent] => 210 [description] => [name] => Mobile Phase Bottles FAQ [name] => AppNotes AgroChem [taxonomy] => category [144] => WP_Term Object They have become popular due to their high mass transfer properties and short purification times. ) [84] => WP_Term Object ) [taxonomy] => category [description] => [parent] => 5 [164] => WP_Term Object [82] => WP_Term Object 13 February 2014. [term_taxonomy_id] => 146 ( ( [filter] => raw ( ) ( [count] => 14 [term_id] => 222 [term_taxonomy_id] => 3 [parent] => 120 [24] => WP_Term Object [term_group] => 0 [count] => 7 [105] => WP_Term Object [count] => 11 [slug] => 171 [filter] => raw [term_taxonomy_id] => 152 [taxonomy] => category [description] => [term_group] => 0 [parent] => 73 [taxonomy] => category [taxonomy] => category [37] => WP_Term Object [term_taxonomy_id] => 231 [description] => [taxonomy] => category [taxonomy] => category [parent] => 105 [name] => General Information [taxonomy] => category [158] => WP_Term Object [taxonomy] => category ( . ) [taxonomy] => category ) [name] => Trouble Shooting Suggestions [parent] => 4 [count] => 9 [description] => [term_taxonomy_id] => 8 [name] => Cogent HPS Column Information ) [count] => 32 [description] => [term_id] => 284 [term_group] => 0 [filter] => raw [term_id] => 227 [term_id] => 127 [description] => [name] => Cogent Silica-C Information [parent] => 5 [143] => WP_Term Object [slug] => 25 [parent] => 271 [term_taxonomy_id] => 99 [slug] => 95 [parent] => 119 [count] => 2 [taxonomy] => category [filter] => raw [name] => AppNotes Normal Phase [parent] => 128 [slug] => 104 [slug] => 37 Then, it was separated by the reversed-phase analytical column. [addMiscTitle] => ) [term_group] => 0 [slug] => 181 [filter] => raw [description] => [term_id] => 111 ( Flush with HPLC grade water until the effluent returns to neutral. [66] => WP_Term Object [filter] => raw ( [description] => [102] => WP_Term Object [title_link] => [parent] => 4 [slug] => rsa-family-of-vials ( [term_group] => 0 Reversed Phase Columns (C18 Columns) InertSustain C18. [parent] => 139 [parent] => 15 [parent] => 7 [name] => Cogent Column Filters - FAQ ( [parent] => 253 [term_id] => 71 [name] => Cogent Diamond Hydride Information ) [taxonomy] => category [description] => ) [name] => AppNotes Cogent Silica-C [filter] => raw High flow rates work better for particulate matter because it may require more force to remove particles that have become trapped in the column frit. [term_id] => 244 [filter] => raw [filter] => raw [count] => 25 [parent] => 119 ( Forms [term_id] => 165 [term_id] => 120 [taxonomy] => category [parent] => 253 [term_group] => 0 [parent] => 76 [parent] => 119 [description] => [name] => Partisil & Partisphere FAQ SOP for Washing of HPLC Columns : Pharmaguideline [128] => WP_Term Object [count] => 50 ( [parent] => 153 [term_id] => 258 [description] => [name] => Mobile Phase Suggestions You need at least 2 of them. ) [slug] => beta-tool-information Normal Phase HPLC - an overview | ScienceDirect Topics [count] => 17 [description] => [taxonomy] => category [filter] => raw [slug] => 28 [slug] => 39 [count] => 40 [name] => U-2D Sample MGT FAQ Disconnect the tubing from the column and reattach the inlet tubing to the outlet of the column. [count] => 79 ) ( [term_id] => 265 - Disconnect the guard column if you are using one. [106] => WP_Term Object [name] => Syringe Filters and Filtration Information This can be small particles of dust of other debris and can come from many different sources such as the sample or HPLC system components. [term_id] => 257 Set the flow rate ( 20 to 50% of the usual one) Rinse with 10 column volumes of each of the following solvents. [description] => [term_taxonomy_id] => 276 [count] => 160 [term_group] => 0 [description] => [term_id] => 274 ( [description] => [slug] => 22 [slug] => 12 [taxonomy] => category [filter] => raw [name] => AppNotes Metabolites [term_group] => 0 PDF Determination of Cobalt in Natural Water as a 2-(5-Bromo-2- pyridylazo [count] => 72 ( ( [filter] => raw [filter] => raw [filter] => raw [term_id] => 81 ) [taxonomy] => category [count] => 1 [name] => AppNotes Cogent Biphenyl [name] => AppNotes Cogent Diol [term_taxonomy_id] => 218 [term_group] => 0 ) [parent] => 76 [parent] => 84 [taxonomy] => category [filter] => raw [name] => Sales Department Outbound [description] => [term_group] => 0 [filter] => raw [term_id] => 276 The Backflush, what is it for, how does it work - CHEMLYS [name] => Amide FAQ [filter] => raw [parent] => 76 [slug] => 90 [term_taxonomy_id] => 149 [149] => WP_Term Object [parent] => 5 [description] => [parent] => 225 [accordion] => 1 [taxonomy] => category [count] => 26 [term_id] => 81 [description] => [name] => AppNotes TYPE-C Columns [name] => Cogent Column Filters - FAQ [173] => WP_Term Object [count] => 421 [term_id] => 287 [parent] => 75 [filter] => raw [taxonomy] => category [description] => [slug] => 53 [description] => The choice of wash solvent will depend on the type of contaminant that is suspected to be present. 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[term_taxonomy_id] => 230 Liquid Chromatography | HPLC Systems | KNAUER ) [term_group] => 0 [count] => 18 If a C18 column is used for a routine analysis and the pressure has risen, as in this example, if the column is reversed and unplugged will this be ok or will be the column be damaged? [term_group] => 0 [term_id] => 168 ( [slug] => 142 [taxonomy] => category ( [name] => VOIXIS Phone Internal postsToExclude: [name] => AppNotes Reversed Phase Methods [parent] => 5 [filter] => raw These particles are generally larger than the column inlet frit pores and therefore become trapped. [count] => 61 [count] => 11 [filter] => raw [parent] => 250 [term_taxonomy_id] => 219 ) [term_id] => 103 [term_taxonomy_id] => 140 [term_group] => 0 [term_id] => 69 These particles are usually held up by the inlet frit which causes a disrupted flow pattern. [description] => [parent] => 7 ( [173] => WP_Term Object [80] => WP_Term Object [filter] => raw Formulation design and material characterization. [count] => 5 [term_group] => 0 [filter] => raw ( [filter] => raw [55] => WP_Term Object [count] => 287 [slug] => 125 [description] => [description] => ( These particles are generally larger than the column inlet frit pores and therefore become trapped. ( ) [term_taxonomy_id] => 285 [slug] => sparging-stone-information [count] => 46 Monitor system pressure without an HPLC column in line. [description] => [term_taxonomy_id] => 293 [filter] => raw ) [filter] => raw [81] => WP_Term Object [slug] => 117 [term_id] => 74 [description] => [slug] => 63 [term_taxonomy_id] => 241 [filter] => raw Reverse the flow direction in the column. [slug] => 82 [term_id] => 236 [description] => [taxonomy] => category - Do not connect the column to your detector. [term_id] => 122 as your HPLC columns - Agilent! [filter] => raw [term_taxonomy_id] => 107 [count] => 25 [name] => Guard Column Information [filter] => raw [parent] => 71 [filter] => raw [parent] => 210 ) [parent] => 4 [description] => [taxonomy] => category The thermodynamically stable and kinetically inert Co(III) chelate Phone:+1 732 380 8900 [term_taxonomy_id] => 300 [count] => 3 [term_group] => 0 [taxonomy] => category [parent] => 298 HILIC Troubleshooting | Thermo Fisher Scientific - US ) [parent] => 128 ) [name] => Cogent HPS FAQ [parent] => 84 [term_id] => 126 [term_taxonomy_id] => 96 [taxonomy] => category [filter] => raw [name] => Column Hardware FAQ [parent] => 4 [parent] => 210 [term_taxonomy_id] => 146 [term_id] => 233 To rejuvenate column performance, follow the reverse flow column flush procedure below: 1. [term_taxonomy_id] => 160 ) [description] => [taxonomy] => category [filter] => raw Technical Notes VII. Pump a 20% nitric acid/water solution through the pump for 3 minutes. Flush it with water for at least 30 min. [filter] => raw [65] => WP_Term Object [filter] => raw How to Clean an HPLC Column - Reverse Flush Method - YouTube [parent] => 120 [useAjax] => 0 What Is Endcapping in HPLC Column - Chrom Tech [term_id] => 14 [slug] => uv-vis-qualification [taxonomy] => category [taxonomy] => category [term_id] => 97 ( This can be small particles of dust of other debris and can come from many dierent sources such as the sample or HPLC . [filter] => raw [term_taxonomy_id] => 84 ( [count] => 130 ( [parent] => 210 [taxonomy] => category [term_id] => 163 [term_id] => 271 [term_id] => 15 [filter] => raw [description] => [taxonomy] => category [term_id] => 207 [count] => 214 [taxonomy] => category [taxonomy] => category ) High pressure in the HPLC system - Forum - Agilent Community [slug] => other-columns-information [parent] => 119 [count] => 1 [count] => 17 [term_taxonomy_id] => 78 [term_taxonomy_id] => 102 ( [taxonomy] => category [name] => Qualification Tech Information Connect the column to the chromatograph so that the flow is in the proper direction. [133] => WP_Term Object [term_group] => 0 [count] => 21 [slug] => 97 [taxonomy] => category [term_taxonomy_id] => 69 ( ) [count] => 5 Back flushing an HPLC column can be done to remove contaminants from an HPLC column without damaging it. [description] => [parent] => 4 [term_id] => 159 Flush column with strong mobile phase at end of each run (see 3.1) 3. [term_id] => 128 [slug] => anp-aqueous-normal-phase-articles [count] => 1 ) [name] => General Policies & Procedure Internal [term_group] => 0 [term_taxonomy_id] => 236 [count] => 18 [description] => ) ( [110] => WP_Term Object [count] => 35 [taxonomy] => category ) [term_taxonomy_id] => 261 [taxonomy] => category [olderThan] => 0 [term_taxonomy_id] => 300 [slug] => buffers-discontinued [153] => WP_Term Object [taxonomy] => category [76] => WP_Term Object [name] => AppNotes Listed by Compound [slug] => 115 [35] => WP_Term Object [161] => WP_Term Object [parent] => 105 If you want to reverse-flush, just reconfigure the column. [taxonomy] => category [slug] => 125 [description] => [count] => 130 [term_group] => 0 [term_group] => 0 [taxonomy] => category [count] => 31 [term_group] => 0 [term_id] => 288 Xinyu Sun - Innovation Scientist - Beyond Meat | LinkedIn [35] => WP_Term Object [count] => 269 [count] => 2 ) Caring for your HPLC column might be simple, but it is exceptionally important to the health, lifetime, and productivity of your column. [10] => WP_Term Object ( [count] => 1 [filter] => raw [name] => U-2D Sample Management System [parent] => 84 [57] => WP_Term Object [count] => 6 [term_id] => 167 [151] => WP_Term Object ( Replace the column with a union. ( [109] => WP_Term Object [description] => The rinse solution used to wash the pistons will again depend on the type of mobile phase you are using (just like the HPLC flushing solvent). [180] => WP_Term Object [count] => 96 [term_id] => 153 [term_taxonomy_id] => 294 [slug] => flanging-tool-information [term_group] => 0 [term_id] => 156 ( [count] => 81 [49] => WP_Term Object ( [count] => 21 If this is the case, column reversal will displace this material perhaps a third of the time, causing the pressure to go back to normal. ( [filter] => raw [term_id] => 220 [slug] => 12 [showPages] => 0 ) [name] => Sales Order Module [term_group] => 0 [slug] => rsa-glass-vial-applications [count] => 80 [term_group] => 0 [filter] => raw [description] => [15] => WP_Term Object [slug] => 96 After a short flushing step, the reduced bsAb1 subunits were eluted by multiple gradients up to 45% eluent B within 50 min. . [count] => 72 [count] => 78 [description] => [term_group] => 0 [slug] => 124 [parent] => 84 [slug] => cogent-rp-column-information [taxonomy] => category ( [term_id] => 239 [term_group] => 0 [description] => [filter] => raw [term_taxonomy_id] => 137 [163] => WP_Term Object ( Phases: C18 pH Range: 1.5-9.0 [slug] => 34 [count] => 42 [118] => WP_Term Object [slug] => 52 [count] => 11 [parent] => 120 [description] => [count] => 2 [filter] => raw ) [32] => WP_Term Object [slug] => metal-free-column-surfaces [description] => ( [taxonomy] => category [taxonomy] => category [description] => [slug] => 72 [count] => 19 [8] => WP_Term Object [term_group] => 0 [count] => 11 ( [filter] => raw ( [term_taxonomy_id] => 153 [description] => [term_group] => 0 [taxonomy] => category [filter] => raw [showPages] => 0 ( [taxonomy] => category [slug] => 129 [term_group] => 0 [term_group] => 0 [description] => [term_id] => 100 [filter] => raw ( [slug] => 155 [filter] => raw [slug] => 11 [customCollapse] => [slug] => 99 [term_id] => 291 to remove air bubbles. [slug] => 41 [taxonomy] => category [term_id] => 221 [slug] => 82 ) [count] => 10 [term_taxonomy_id] => 129 [description] => [term_group] => 0 ) [term_id] => 101 [taxonomy] => category [slug] => chrom-syringes-faq [name] => Window Maker FAQ [18] => WP_Term Object [term_group] => 0 ( [name] => AppNotes Listed by Discipline [count] => 57 In this article, we consider whether it is ok to back-flush a column. [term_id] => 257 [term_id] => 298 [term_id] => 286 [term_group] => 0 [description] => [description] => [term_group] => 0 ( [count] => 11 ( [taxonomy] => category [taxonomy] => category [term_group] => 0 [description] => [term_taxonomy_id] => 244 [name] => Cogent Diamond Hydride Information [description] => [parent] => 14 [name] => Cogent UDC-Cholesterol Information [parent] => 210 [filter] => raw [term_group] => 0 [term_taxonomy_id] => 263 [term_group] => 0 [term_taxonomy_id] => 234 [filter] => raw [5] => WP_Term Object Array ) [70] => WP_Term Object [filter] => raw [slug] => 138 [term_taxonomy_id] => 168 [parent] => 0 [slug] => 19 [count] => 57 [description] => [name] => UV-vis Qualification [term_id] => 218 [term_taxonomy_id] => 147 [term_group] => 0 [parent] => 120 ( [92] => WP_Term Object [term_group] => 0 [term_id] => 281 ) [count] => 79 [slug] => 22 - Disconnect the column and install it in the reverse direction. [118] => WP_Term Object [term_group] => 0 [term_group] => 0 POST QUERY RESULTS. ( PDF What is the purpose of back flushing an HPLC column and will it damage [slug] => 98 [count] => 1 [slug] => 144 ) [slug] => 73 [142] => WP_Term Object [slug] => cogent-bidentate-c18-information [description] => [term_id] => 125 ( [term_taxonomy_id] => 292 [slug] => 92 [filter] => raw [count] => 24 [parent] => 225 [term_taxonomy_id] => 128 [slug] => phenyl-hydride-faq [description] => [taxonomy] => category [term_id] => 242 [description] => [slug] => 111 HPLC PUMP SEAL WASH & FLUSHING THE HPLC - Blogger [parent] => 0 [slug] => 10 [term_taxonomy_id] => 257 [taxonomy] => category [term_group] => 0 [taxonomy] => category POST QUERY RESULTS. [name] => Partisil & Partisphere FAQ ) [taxonomy] => category [description] => ( Ron Majors also discusses cleaning procedures for bonded-silica and other types of reversed-phase columns. [term_id] => 138 [term_id] => 103 [slug] => pricing-policies ) ( [description] => [description] => [filter] => raw ) [postSort] => postTitle ( [82] => WP_Term Object [style] => kubrick [count] => 1 [filter] => raw [description] => [description] => [slug] => metal-free-column-surfaces [description] => [term_group] => 0 ( [filter] => raw [term_group] => 0 [term_group] => 0 ( [slug] => 166 [slug] => autosampler-vial-tips [term_group] => 0 [taxonomy] => category [useAjax] => 0 [parent] => 210 ( [parent] => 210 [name] => Capillary Electrophoresis Information Guidelines for Routine Use and Maintenance of Ultra-Performance Size SOP for HPLC Column Maintenance and Washing Procedure [filter] => raw [filter] => raw [parent] => 210 3. Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. [term_id] => 297 [term_group] => 0 ) [term_group] => 0 [filter] => raw [filter] => raw [count] => 16 [term_group] => 0 [slug] => 116 ( [parent] => 210 [name] => Pricing Policies ( Do reverse phase HPLC columns really need conditioning? [description] => ( [parent] => 147 [term_id] => 228 [parent] => 0 [term_group] => 0 Reverse flushing helps with all those issues, especially for particulate build-up. If your intent is to switch to 100% organic solvent, make sure you flush out the buffer first using a mix that is at least 50% water for at least 7 column volumes. ) [taxonomy] => category [filter] => raw [count] => 11 [description] => [term_id] => 171 ) [filter] => raw [term_id] => 119 ( [taxonomy] => category [name] => Labelled Vials ( [description] => [parent] => 271 [name] => Autosampler Vials Information [term_group] => 0 [filter] => raw [name] => HPLC Primer [84] => WP_Term Object [term_taxonomy_id] => 224 ) [slug] => 86 ( [term_id] => 109 [taxonomy] => category [name] => Headspace Vial FAQ [name] => HPLC Suggestions and Information [term_id] => 165 [name] => Bidentate C18 FAQ [filter] => raw [term_id] => 142 [count] => 411 [term_group] => 0 [term_id] => 17 [term_taxonomy_id] => 209 [slug] => pricing-policies [term_id] => 272 [slug] => guard-column-information ) [filter] => raw [parent] => 139 Supel Carbon LC U/HPLC Columns . [term_taxonomy_id] => 83 [parent] => 76 [count] => 109 [term_id] => 129 [103] => WP_Term Object [slug] => 14 [filter] => raw [parent] => 153 [name] => Qualification FAQ [108] => WP_Term Object [term_taxonomy_id] => 138 for 10 minutes. ) [name] => AppNotes Cogent Silica-C [name] => HPLC & GC Standard Vials Caps & Inserts [slug] => autosampler-vial-tips To remove strongly retained materials from the column, flush the column in the reverse direction with very strong solvents such as 50:50 methanol:water. ( [term_group] => 0 [term_group] => 0 [term_taxonomy_id] => 218 [taxonomy] => category [term_group] => 0 [name] => SAGE 100 [term_id] => 233 [slug] => 43 [count] => 115 [2] => WP_Term Object 5.2.2 Allow mobile phase to flow through the column at the rate of 1 ml/ min. Extend run time (see 3.1) 2. The pre-column was used to concentrate the terbutaline in the eluent from the C18 column before back-flushing onto the silica column with fluorescence detection at an excitation/emission wavelength of 276/306 nm. [description] => [85] => WP_Term Object [name] => AppNotes Cogent HPS [parent] => 153 [5] => WP_Term Object [count] => 11 [description] => [65] => WP_Term Object [filter] => raw ( Size Exclusion HPLC Columns [term_taxonomy_id] => 207 [58] => WP_Term Object [name] => Accounts Receivable ) [128] => WP_Term Object If not re-tighten and keep going backwards, so to the next connection. Array [17] => WP_Term Object ) [term_group] => 0 [filter] => raw [term_taxonomy_id] => 272 [slug] => fittings-connectors-nuts-ferrules-information [term_group] => 0 [term_group] => 0 [term_id] => 301 Before you install a column, replace the old one with a union connector and let the system go through a run cycle. [description] => ) [term_taxonomy_id] => 291 [term_group] => 0 [36] => WP_Term Object USP tailing factor T A tailing peak has a front of greater than 1.0, while a fronting peak has a front of less than 1.0. [description] => [parent] => 71 [filter] => raw [filter] => raw Be sure that the column outlet is disconnected from the rest of the system or the particles may end up in the detector and cause damage. ) [term_group] => 0 [172] => WP_Term Object [29] => WP_Term Object ) [term_id] => 241 [taxonomy] => category [term_taxonomy_id] => 121 [filter] => raw [term_group] => 0 [term_group] => 0 ( [count] => 0 The ASTM International standards organization recommends calculating column symmetry or asymmetry (A s) as the back-to-front ratio of a bisected peak measured at 10% of height. [filter] => raw [filter] => raw [term_taxonomy_id] => 124 [taxonomy] => category [slug] => 127 ( [parent] => 76 [count] => 4 [filter] => raw [name] => Packaging Department [taxonomy] => category [term_group] => 0 ) [filter] => raw [106] => WP_Term Object [parent] => 232 [parent] => 75 [slug] => 137 ) [term_group] => 0 [3] => WP_Term Object [taxonomy] => category ( [term_id] => 7 [parent] => 205 [term_taxonomy_id] => 70 [parent] => 211 [term_id] => 255 ) ( [74] => WP_Term Object [term_group] => 0 [count] => 24 [count] => 7 ( [count] => 11 [slug] => 127 [31] => WP_Term Object [term_id] => 230 [description] => [postsBeforeCats] => 0 [term_taxonomy_id] => 297 ( [6] => WP_Term Object [slug] => 77 [term_id] => 100 [name] => Sales Department Inbound [slug] => 166 [96] => WP_Term Object [134] => WP_Term Object [description] => [accordion] => 1 [filter] => raw [slug] => hplc-primer [slug] => cogent-diamond-hydride [term_id] => 17 ( [filter] => raw ) [parent] => 139 [term_id] => 139 [slug] => 144 [parent] => 84 [term_id] => 269 [taxonomy] => category ) This can be small particles of dust of other debris and can come from many different sources such as the sample or HPLC system components. [count] => 9 [term_taxonomy_id] => 282 ( ( [term_taxonomy_id] => 286 [term_id] => 230 [slug] => 122 [description] => [slug] => plastic-vials [slug] => reversed-phase-columns-faq [taxonomy] => category [slug] => u-2d-sample-management-system The solvent is drawn into a small chamber (with the solvent check valve open) and pumped out of it (when the column check valve is open) by the back and forth motion of a motor driven piston. [count] => 74 [name] => Qualification Information [description] => [term_group] => 0 Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. [term_group] => 0 ) [parent] => 153 [count] => 141 [filter] => raw [term_id] => 71 [2] => WP_Term Object [taxonomy] => category [filter] => raw [term_group] => 0 [taxonomy] => category [term_taxonomy_id] => 283 [term_group] => 0 [name] => Mobile Phase Suggestions [defaultExpand] => [term_id] => 104 [taxonomy] => category [131] => WP_Term Object [term_group] => 0 [term_id] => 263 [term_id] => 7 [description] => ) Lines primed (no dry lines or bubbles) 3. ) ) [description] => [term_taxonomy_id] => 226 Flush column to remove impurities (see 5.2) 3. [term_id] => 57 [parent] => 4 [term_taxonomy_id] => 81 [taxonomy] => category ) The types of contaminants can come from many sources as well, but the most common is when a complex sample matrix is injected (e.g. [parent] => 76 ( ( [taxonomy] => category [filter] => raw ( However, if your column is so clogged that it can't flow at all, flip the column and run it in the opposite direction. [165] => WP_Term Object [description] => [taxonomy] => category [name] => Inventory Module [description] => ) [slug] => 27 [filter] => raw [parent] => 4 [count] => 4 Simply reverse the column and flush to waste. [filter] => raw ) [taxonomy] => category ( [parent] => 153 [filter] => raw DEC 6 . [term_id] => 2 It might be time to flush your HPLC column when you're seeing high back pressure, low efficiency, split peaks, or the most common issue, particulate build-up at the head of the column. [description] => . [name] => ANP Aqueous Normal Phase Articles [taxonomy] => category How to Clean and Unclog Your HPLC Column - Bitesize Bio [count] => 25 [123] => WP_Term Object NOV 30. [term_group] => 0 [slug] => 75 [count] => 17 [name] => Technical Support Dept Internal [description] => ( [count] => 10 [term_taxonomy_id] => 250 [count] => 5 [slug] => 114 [count] => 3 [filter] => raw [showPostDate] => 0 ( [name] => MTCP [filter] => raw [name] => SFDC Salesforce.com [parent] => 14 [count] => 10 [taxonomy] => category [name] => HPLC Tips and Suggestions [taxonomy] => category Your columns health . ) ) [155] => WP_Term Object [slug] => appnotes-usp-methods [term_id] => 260 [description] => [description] => ( [slug] => cogent-hps-rp-and-np-column-information [term_group] => 0 [inExcludeCats] => default-title,uncategorized,featured-articles,180,1651,ce-accessories [124] => WP_Term Object [catSortOrder] => ASC ( [150] => WP_Term Object [term_id] => 261 [description] => [name] => AppNotes Cogent Amide [parent] => 105 [term_group] => 0 [98] => WP_Term Object ( [taxonomy] => category [filter] => raw [slug] => di-ad-column-connectors [description] => [parent] => 4 ( [110] => WP_Term Object [90] => WP_Term Object [name] => Syringe Filters FAQ [name] => Accounting Dept. [174] => WP_Term Object Initial setup of your LC system is very important to ensure column performance: Ensure that your LC system is ready: 1. [description] => [term_group] => 0 [taxonomy] => category [50] => WP_Term Object [6] => WP_Term Object ) [term_taxonomy_id] => 15 The reason for this is so any contaminants coming off the Column don't end up in the Detector and damage it. [filter] => raw [filter] => raw [term_group] => 0 [count] => 12 ( Direct the column outlet to waste, not to the tubing leading to the detector. [filter] => raw ( [slug] => di-ad-column-connectors [term_group] => 0 [57] => WP_Term Object [name] => ONLINE TOOLS [name] => Reversed Phase Columns FAQ [parent] => 271 [filter] => raw ) [filter] => raw [filter] => raw [taxonomy] => category [filter] => raw [slug] => cogent-diol-information HPLC Column Care, Storage, and Restoration - Hamilton Company [term_taxonomy_id] => 18 [taxonomy] => category [parent] => 120 [count] => 96 [name] => AppNotes Listed by Discipline [term_group] => 0 [parent] => 210 [taxonomy] => category [162] => WP_Term Object ( [parent] => 208 [filter] => raw [parent] => 224 [count] => 192 [filter] => raw [62] => WP_Term Object [term_id] => 73 [description] => [term_id] => 111 [term_id] => 232 How to make HPLC columns last longer? - PharmaSky [term_id] => 120 [term_id] => 274 [term_group] => 0 [filter] => raw [term_taxonomy_id] => 107 [term_taxonomy_id] => 253 [name] => Vials & Caps General FAQ ) [term_group] => 0 [name] => Technical Support Dept Internal ( [description] => [term_group] => 0 [term_id] => 264 [count] => 14 [53] => WP_Term Object [filter] => raw [slug] => 14 [count] => 141 [filter] => raw [slug] => sample-loops [term_id] => 249 [debug] => 1 [name] => Cogent UDC-Cholesterol Information ( [24] => WP_Term Object [count] => 11 [term_taxonomy_id] => 114 [slug] => 61 Back flush column - may clear plugged column inlet frit ) ( [slug] => window-maker-information [taxonomy] => category [description] => [term_taxonomy_id] => 119 [filter] => raw [filter] => raw [description] => [slug] => 23 [term_group] => 0 [term_taxonomy_id] => 162 [count] => 4 [showPosts] => 0 ) [count] => 1 [term_taxonomy_id] => 214 [count] => 12 [term_group] => 0 ) [name] => AppNotes Cogent Bidentate C18 [61] => WP_Term Object [slug] => 15 [name] => HPLC Columns FAQ [taxonomy] => category [expandCatPost] => 1 [term_taxonomy_id] => 242 [parent] => 253 [count] => 17 [slug] => 138 [parent] => 105 [term_id] => 219 ) [parent] => 84 [11] => WP_Term Object [term_id] => 212 [slug] => 39 [term_id] => 280 ) [slug] => sparging-stone-information ) [name] => AppNotes Chemicals/Energy [taxonomy] => category [count] => 218 [parent] => 14 [term_taxonomy_id] => 158 [count] => 7 [taxonomy] => category [parent] => 120 [filter] => raw [slug] => chrom-syringes-faq [description] => [count] => 5 [slug] => 27 ( [term_group] => 0 [slug] => hplc-and-gc-vials-caps-inserts [term_taxonomy_id] => 171 [description] => [count] => 61 The second type is chemically adsorbed contaminants. [count] => 50 [description] => [term_id] => 302 [40] => WP_Term Object [term_id] => 72 Fax:+1 910 769 9435 [taxonomy] => category [150] => WP_Term Object [name] => AppNotes Cogent Bidentate C8 300 [filter] => raw [parent] => 7 [taxonomy] => category [slug] => 48 [parent] => 5 ) [description] => [count] => 20 [slug] => 74 [filter] => raw Reversed-Phase Columns. [name] => Equipment FAQ [name] => VOIXIS Phone Internal [description] => [filter] => raw [term_taxonomy_id] => 102 [slug] => mobile-phase-bottle-information [parent] => 71 [slug] => 18 . [filter] => raw [term_taxonomy_id] => 18 [parent] => 119 [112] => WP_Term Object HPLC Pump Care and Troubleshooting - Chrom Tech [filter] => raw [term_taxonomy_id] => 139 [count] => 40 [count] => 76 Sreanje uporabnikov in demo predstavitev Seahorse XF intrumentov 2022. [description] => [count] => 7 [term_taxonomy_id] => 272 [term_taxonomy_id] => 84 [term_id] => 254 [170] => WP_Term Object [term_group] => 0 [taxonomy] => category [term_id] => 224 [87] => WP_Term Object [taxonomy] => category [taxonomy] => category [count] => 75 [slug] => 120 [parent] => 15 [term_group] => 0 [term_id] => 208 [term_taxonomy_id] => 100 Flush the column with 5 % water and 95 % Acetonitrile for 15 minutes. [56] => WP_Term Object [description] => [name] => Photochemical Reactor Internal [description] => [filter] => raw [143] => WP_Term Object [term_taxonomy_id] => 3 ( [term_taxonomy_id] => 284 In a first step, this guard column will retain the undesirable compounds and send only the permanent gases into the MS5A. [taxonomy] => category [taxonomy] => category ) [43] => WP_Term Object Toll-free USA:1 888 248 4972 [name] => Financial Reporting [name] => HPLC Column Information [description] => [filter] => raw [parent] => 75 [name] => Cogent Phenyl Hydride Information [filter] => raw [114] => WP_Term Object [filter] => raw [term_taxonomy_id] => 240 [slug] => 77 [description] => [parent] => 119 [description] => Flush Reversed-Phase Column Forward use and back flushing Since most of the contaminants which have strong retention ability usually appear at the column-head, therefore, back flushing can greatly reduce the distance that contaminants need for leaving column. [name] => Diamond Hydride FAQ [taxonomy] => category [125] => WP_Term Object [term_taxonomy_id] => 220 [term_group] => 0 [count] => 63 ( [term_group] => 0 [slug] => 53 [name] => Tools Information [taxonomy] => category [taxonomy] => category ( [filter] => raw [taxonomy] => category [term_taxonomy_id] => 256 [taxonomy] => category [description] => [parent] => 5 ( Direct the Column Outlet to Waste, not to the tubing leading to the detector. [filter] => raw [term_id] => 162 [37] => WP_Term Object [description] => [slug] => uv-vis-qualification [slug] => vial-centrifuge [count] => 2 [taxonomy] => category ( [term_group] => 0 [slug] => 99 [excludeAll] => 0 [count] => 3 ( [taxonomy] => category [name] => Trouble Shooting Suggestions [parent] => 71 [term_taxonomy_id] => 206 [47] => WP_Term Object [excludeAll] => 0 The other is that an HPLC column with a collapsed HPLC column head is not suitable for backflushing, because the HPLC column head collapses to leave space on the HPLC column head. ) [parent] => 4 [name] => Employee Cell Phone Internal [taxonomy] => category ) ( Atlantis Silica HILIC Column, 100, 3 m, 2.1 mm X 30 mm, 1/pkg. [27] => WP_Term Object [parent] => 84 [term_taxonomy_id] => 76 ) [slug] => rsa-family-of-vials [parent] => 120 [parent] => 84 [count] => 84 [term_taxonomy_id] => 263 [count] => 2 [parent] => 5 [taxonomy] => category [count] => 27 [term_taxonomy_id] => 282 [name] => CE and CZE FAQ [taxonomy] => category [162] => WP_Term Object [filter] => raw [term_id] => 75 [slug] => appnotes-cogent-phenyl-hexyl ( [term_group] => 0 [taxonomy] => category For columns with a smaller particle size, the pore size should be 0.2 m. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. Article No. [parent] => 15 Page 2 Goals for this presentation: 1. [parent] => 205 [term_id] => 299 [taxonomy] => category ( [term_id] => 110 [term_id] => 266 [59] => WP_Term Object [count] => 38 [count] => 10 [count] => 22 [count] => 25 [filter] => raw ) [description] => ( [parent] => 4 ( [term_taxonomy_id] => 144 [filter] => raw - Set the flow rate to .1mL/min for 2.1-mm ID columns or .3mL/min for 3.0-mm ID columns. [taxonomy] => category [filter] => raw [filter] => raw [term_taxonomy_id] => 164 [term_taxonomy_id] => 99 [term_taxonomy_id] => 230 [filter] => raw [parent] => 15 [count] => 9 [term_group] => 0 [count] => 128 [157] => WP_Term Object ( [name] => HPLC Qualification [30] => WP_Term Object [description] => [term_group] => 0 [term_group] => 0 [description] => [term_group] => 0 [127] => WP_Term Object [slug] => 114 [count] => 21 [count] => 25 [collapseSym] => How to maintain HPLC column? Here you are! Welch Materials [taxonomy] => category [filter] => raw [parent] => 271 [filter] => raw [filter] => raw ) [count] => 8 [term_group] => 0 [filter] => raw [term_id] => 253 ) [term_group] => 0 [description] => [135] => WP_Term Object [term_group] => 0 [count] => 5 [parent] => 210 [term_group] => 0 [description] => [filter] => raw [parent] => 75 ( [91] => WP_Term Object ( [name] => Cogent Bidentate C8 Information ) [term_taxonomy_id] => 222 Definition: Having power to compel or constrain , power to retain and resolve. [filter] => raw ( [taxonomy] => category [term_id] => 3 [term_id] => 293 [parent] => 4 [filter] => raw ( ( [term_id] => 210 [47] => WP_Term Object ) [parent] => 5 [slug] => 168 Flushing and Regeneration of Reversed-Phase Column [78] => WP_Term Object [parent] => 224 [88] => WP_Term Object ) ( [term_id] => 277 [name] => Plastic Vials [term_group] => 0 [term_taxonomy_id] => 265 ( [176] => WP_Term Object 5.1.6 Disconnect the column from the HPLC unit and store in an appropriate place. [count] => 26 ) [taxonomy] => category [description] => [parent] => 84 [taxonomy] => category [filter] => raw ( ( [term_taxonomy_id] => 232 [term_id] => 262 We blamed the column manufacturer at first, so switched to another column from Agilent, and it still happened. [term_group] => 0 [count] => 4 [name] => Guard Column Information [term_taxonomy_id] => 142 [description] => ) ( [description] => ) [taxonomy] => category [name] => Online Book of Standards [count] => 6 [slug] => 8 [count] => 177 [term_group] => 0 [name] => Purchasing Department [term_taxonomy_id] => 144 [taxonomy] => category [term_taxonomy_id] => 287 [taxonomy] => category [term_id] => 99 [term_taxonomy_id] => 221 [slug] => appnotes-cogent-phenyl-hexyl ) [slug] => cogent-udc-cholesterol-information ) ) ( [name] => Syringe Filters and Filtration Information [taxonomy] => category [description] => [term_id] => 263 [slug] => plastic-vials [parent] => 225 [53] => WP_Term Object [83] => WP_Term Object ) [term_id] => 242 [filter] => raw [term_group] => 0 [parent] => 139 ) [term_id] => 292 [count] => 3 ( [description] => PDF HPLC Troubleshooting Guide [taxonomy] => category [slug] => 130 [term_taxonomy_id] => 135 [description] => [term_group] => 0 ) [description] => 6. [term_taxonomy_id] => 212 [description] => ) [taxonomy] => category [term_group] => 0 [77] => WP_Term Object [name] => Warehousing [taxonomy] => category [name] => U-2D Sample MGT FAQ [term_id] => 275 ( ) [filter] => raw [slug] => sample-loops [term_id] => 215 [parent] => 225 [term_id] => 223 [term_taxonomy_id] => 127 [name] => Tubing Information [term_id] => 164 ) [description] => [term_group] => 0 [term_group] => 0 [count] => 19 [description] => ) [filter] => raw ( [taxonomy] => category [count] => 3 [name] => Window Maker Information ) [taxonomy] => category [taxonomy] => category ( [term_group] => 0 Maintain the column at (55 o C) in the column oven during flushing. ( [taxonomy] => category [name] => Mobile Phase FAQ [term_id] => 146 [term_group] => 0 ) [slug] => beta-tool-information [filter] => raw [term_id] => 290 [term_id] => 145 ( [slug] => 86 [description] => [count] => 17 [parent] => 153 [count] => 18 ( [count] => 75 [parent] => 210 [term_taxonomy_id] => 109 [slug] => 100 [139] => WP_Term Object [158] => WP_Term Object [filter] => raw [count] => 21 [term_id] => 76 [34] => WP_Term Object [description] => [count] => 36 [term_taxonomy_id] => 8 ( [slug] => 157 [name] => Flanging Tool Information [parent] => 75 [slug] => 34 [term_id] => 228 [name] => Flanging Tool Information [169] => WP_Term Object [description] => [89] => WP_Term Object ) [filter] => raw [useCookies] => 0 [description] => [term_taxonomy_id] => 240 [term_group] => 0 [63] => WP_Term Object [parent] => 210 ) [name] => RSA Vials FAQ ( [taxonomy] => category [parent] => 210 [term_group] => 0 Deterioration is more evident in higher efficiency columns. [filter] => raw [description] => ) ( [taxonomy] => category [filter] => raw [count] => 10 ( [term_taxonomy_id] => 78 ) [name] => Cogent Bidentate C18 Information [count] => 16 [term_id] => 160 [term_group] => 0 ( [taxonomy] => category [term_group] => 0 [filter] => raw [name] => SALES POLICIES [filter] => raw [slug] => appnotes-cogent-rp-c8 ) ( [term_taxonomy_id] => 97 Nancy Schwartz. [term_taxonomy_id] => 170 [taxonomy] => category [parent] => 210 [slug] => 18 [parent] => 14 [slug] => 131 [parent] => 76 [description] => [filter] => raw [postTitleLength] => 0 [taxonomy] => category For particulate matter, select a higher flow rate. [slug] => 172 [29] => WP_Term Object [name] => Sparging Stones FAQ 3. [count] => 8 [description] => [term_taxonomy_id] => 135 [description] => [name] => AppNotes Library [name] => Employee Cell Phone Internal [taxonomy] => category Sample matrix. [name] => AppNotes RSA Vials ) [term_group] => 0 [description] => [filter] => raw [name] => Di-Ad Column Connector Information [term_id] => 272 ( [parent] => 7 [term_taxonomy_id] => 75 [slug] => 76 [term_taxonomy_id] => 168 Safety Precaution. [term_taxonomy_id] => 299 [slug] => 108 [slug] => 88 [filter] => raw [98] => WP_Term Object Back flush can also be used to clean the column. [name] => Cogent Silica-C Information [name] => AppNotes Food and Beverages ) [term_group] => 0 [filter] => raw [parent] => 4 [166] => WP_Term Object [parent] => 84 [filter] => raw [term_id] => 211 [count] => 9 [filter] => raw For columns with a particle size > 5 m, the pore size of the filter should be 0.45 m. [filter] => raw [parent] => 15 [slug] => celixir-products [term_group] => 0 [name] => Mobile Phase Bottle Information [filter] => raw [filter] => raw [term_taxonomy_id] => 225 ( HPLC Columns Columns and Consumables Edward Kim Applications Engineer January 17, 2008. [term_group] => 0 [taxonomy] => category [term_id] => 270 [term_taxonomy_id] => 228 [slug] => 140 [description] => [slug] => 119 [description] => [name] => Cogent Bidentate C8 Information [28] => WP_Term Object [term_group] => 0 [olderThan] => 0 [description] => [description] => [term_id] => 97 [term_id] => 162 ) (Table 2 lists the column volumes for various sizes of HPLC columns, so readers can easily determine a flush volume for a particular by washing a column with non-aqueous [parent] => 271 [slug] => 158 [term_id] => 256 ) [term_taxonomy_id] => 106 [name] => CElixir Products Internal [42] => WP_Term Object [filter] => raw [term_taxonomy_id] => 73 [filter] => raw [slug] => 146 [parent] => 76 [term_id] => 295 ( ( [count] => 1 [parent] => 5 [116] => WP_Term Object ORDERING INFORMATION Cogent Definition Pronunciation: 'kO-j&nt or Ko Gent. [description] => [description] => [name] => CElixir Products Internal [taxonomy] => category What is the purpose of back flushing an HPLC column and will it damage [45] => WP_Term Object [64] => WP_Term Object [41] => WP_Term Object [taxonomy] => category [term_group] => 0 [description] => [term_taxonomy_id] => 5 [12] => WP_Term Object [description] => [description] => [count] => 6 [count] => 19 [filter] => raw HPLC Column Recycle : 4 Steps - Instructables [taxonomy] => category [term_taxonomy_id] => 73 [filter] => raw [count] => 1 ) [count] => 84 [count] => 3 [term_taxonomy_id] => 226 [129] => WP_Term Object [parent] => 84 [description] => [description] => [term_group] => 0 [term_taxonomy_id] => 74 [description] => [term_taxonomy_id] => 237 [description] => ( ) ) [taxonomy] => category [filter] => raw [taxonomy] => category [slug] => 50 [taxonomy] => category [term_taxonomy_id] => 288 [taxonomy] => category [description] => ) [term_taxonomy_id] => 239 [term_taxonomy_id] => 296 [term_group] => 0 [21] => WP_Term Object [filter] => raw [parent] => 4 [name] => MicroSolv Autosampler Vials FAQ [term_taxonomy_id] => 154 [term_group] => 0 [count] => 9 [name] => Sample Loops [count] => 2 [name] => Qualification Information [term_id] => 285 Application Specific HPLC Columns Designed for key pharmaceutical and environmental applications, these columns utilize novel and unique chemistries to provide superior resolution with ease of use. [parent] => 210 Check this occasionally, as an increase would indicate a blockage or restriction has . [description] => [count] => 29 [term_taxonomy_id] => 217 [description] => [26] => WP_Term Object [slug] => 20 [parent] => 153 [parent] => 76 [filter] => raw For gradient runs, end at higher concentration (see 3.1) [term_taxonomy_id] => 254 [term_id] => 106 [term_group] => 0 [term_id] => 158 [parent] => 14 [term_group] => 0 [parent] => 210 [term_id] => 126 [name] => AppNotes Cogent UDA [term_id] => 124 ( [term_id] => 69 [taxonomy] => category ) [filter] => raw Date: 1659 to Present and for the future.. [description] => [filter] => raw [taxonomy] => category [name] => Qualification Tech Information 1. [slug] => cogent-hps-rp-and-np-column-information [name] => Cogent DIOL Information [term_id] => 264 [description] => [slug] => cogent-silica-c-information [description] => El Segundo, California, United States. [152] => WP_Term Object ( [description] => [parent] => 5 These are strongly retained compounds that usually build up at the head (first 2mm) of the column. [name] => Packaging Department ( [slug] => 24 [term_group] => 0 [taxonomy] => category All Rights Reserved. [slug] => 105 [term_group] => 0 [parent] => 271 [description] => [term_id] => 241 Key Reminders 1. For stainless steel flow paths, proceed to Step 6; For PEEK flow paths, the cleaning procedure is completed. [term_taxonomy_id] => 208 [term_group] => 0 [4] => WP_Term Object ( [term_taxonomy_id] => 69 [description] => [term_id] => 232 [slug] => 145 ( [103] => WP_Term Object [filter] => raw Website & Hosting by, Autosampler Vials, Caps, Inserts & Accessories, Request Troubleshooting and Technical Assistance, Chromatography Accessories & Supplies Information, Fittings Connectors Nuts Ferrules Information, Syringe Filters and Filtration Information, Cleaning Cogent TYPE-C silica based HPLC columns Tips & Suggestions, PFAS and other contaminants leaching into an analysis Tips & Suggestions, What is the purpose of back flushing an HPLC column and will it damage it FAQ, If you cant find what you are looking for, email us, collapsCat options: [ slug ] = > 15 Page 2 Goals for this presentation: 1 minute. 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